Hygiena foodproof STEC Screening LyoKit, 5'Nuclease, Low Profile KIT230077

The foodproof STEC Screening LyoKit provides rapid detection of Shiga toxin-producing E. coli DNA in food and environmental samples. This AOAC-validated real-time PCR kit screens for Shiga toxins (stx1 & stx2) and intimin (eae) genes in enriched cultures from meat, sprouts, and other matrices. Designed for 96 reactions, the kit features a lyophilized PCR mix for enhanced stability and convenience and employs 5' nuclease probe chemistry in a low profile tube format (LP: KIT230077) for use on major real-time PCR platforms. It is validated according to ISO/TS 13136 and intended for food safety testing laboratories.

Key Features:

• Targets: stx1, stx2 and eae genes (Shiga toxin and intimin).
• Technology: real-time PCR with 5' nuclease probe chemistry.
• Reactions: 96 reactions per kit (up to 94 samples plus positive and negative controls).
• Format: lyophilized ready-to-use PCR mix in white low-profile tubes (LP: KIT230077).
• Validation/Certification: AOAC-RI validated and validated according to ISO/TS 13136.
• Compatibility: designed for major real-time PCR instruments with FAM, HEX, ROX and Cy5 detection channels.

Advantages:

• Easy-to-use workflow with few pipetting steps and simplified sample and reagent handling.
• Superior sensitivity capable of detecting small organism numbers with low DNA levels.
• Rapid results that reduce time-to-decision for testing laboratories.
• Proven protocols and broad instrument compatibility for straightforward integration into existing PCR workflows.